Phylogenetic tree constructed by parsimony analyses of the deduced peptide sequences of acetate kinase of available Mollicutes in HAMAP database, Spiroplasma citri, Clostridium botulinum, Erysipelothrix rhusiopathiae, Bacillus subtilis subsp.

subtilis, Lactobacillus plantarum, Enterococcus faecalis, Streptococcus pneumonia, and Lactococcus lactis subsp. lactis employing Escherichia coli and Salmonella typhimurium as outgroup. One of the two most parsimonious trees is shown. Accession numbers are given in parentheses. Numbers on the branches are bootstrap values obtained for 1,000 replicates (only values above 60% are shown). The tree is drawn to scale, with branch lengths calculated using the average pathway method, and are in the units of the number of changes over the whole sequence. The scale bar represents 50 amino acid substitutions.