Phylogenetic analysis of B. pseudomallei, B. mallei, and B. thailandensis strains by bucl-locus typing.

Bayesian analysis was performed on concatenated nucleotide sequences of the non-collagenous regions of bucl3, bucl4, and bucl8 present in a set of 13 B. pseudomallei, 11 B. mallei, and 9 B. thailandensis strains (as shown in Table 3). Support values for each branch are shown as posterior probability from Bayesian analysis and bootstrap values from maximum parsimony analysis, respectively (PP/MP). Posterior probability value which was not supported by maximum parsimony analysis is shown in red. Phylogenetic Clusters 1–4 (C1-C4) correlated with geographic location of B. pseudomallei strains, whereas Clusters 5–7 (C5-C7) contained B. thailandensis strains that made up a separate branch from B. pseudomallei and B. mallei strains. Scale bar is representative of evolutionary distance in substitutions per nucleotide.