Overview of methods and strategies for focused mutagenesis on multiple positions.

Comparison of key performance parameters and experimental requirements of the most prominent and widely used methods for multi site-directed and site-saturation mutagenesis including the here reported OmniChange method.

6 1158-1160, 1162, 1164-115ties,stuft werden lce ingestuftsay entwickelt werden welcher ein rol (negative control/no primers.

*Site Directed Mutagenesis;

**Site Saturation Mutagenesis;

***45 positions targeted; n.r. (not reported);

Limitations or restrictions for distance between targeted codons;

12 phosphorothioated nucleotides on 5′end; a) Linear DNA amplification b) OEP-PCR c) Assembly/nested PCR d) Standard PCR e) PCR with complementary primers.