Figure_1.tif (116.06 kB)
Overexpression of β-catenin-S33Y and Wnt ligans leads to increase in MENA transcription.
figure
posted on 2013-02-20, 05:02 authored by Ayaz Najafov, Tuncay Şeker, İpek Even, Gerta Hoxhaj, Osman Selvi, Duygu Esen Özel, Ahmet Koman, Necla Birgül-İyison(A) β-catenin-S33Y mutant was stably overexpressed in Huh7 cells and MENA and Axin2 mRNA levels were analyzed using qRT-PCR. (B) β-catenin-S33Y was transiently overexpressed (48 hrs) in U373 cells and MENA and β-catenin mRNA levels were compared using RT-PCR. (C) Wnt1 or Wnt3a ligans were overexpressed in Huh7 cells (48 hrs) and MENA mRNA levels were compared using RT-PCR.
