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OncoQuick Enrichment and Recovery of Melanoma CTCs.

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posted on 2013-02-20, 02:13 authored by Gary A. Clawson, Eric Kimchi, Susan D. Patrick, Ping Xin, Ramdane Harouaka, Siyang Zheng, Arthur Berg, Todd Schell, Kevin F. Staveley-O’Carroll, Rogerio I. Neves, Paul J. Mosca, Diane Thiboutot

Left Panel shows a schematic of OncoQuick enrichment. Right Panel shows recovery of SK-MEL-28 human melanoma cells from blood. Known numbers of SK-MEL-28 cells were added to 7.5 ml of blood, and processed using OncoQuick spin columns as described. Recovery of cells was assessed using QPCR amplification of KRT8 and 18 RNAs as described.

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