Figure_3.tif (4.33 MB)

Negative stain electron microscopy reconstruction of HIV SOSIP.664 trimer in complex with Fab PGT122 and in comparison to PGT128

figure

posted on 2013-05-02, 01:50 authored by Jean-Philippe Julien, Devin Sok, Reza Khayat, Jeong Hyun Lee, Katie J. Doores, Laura M. Walker, Alejandra Ramos, Devan C. Diwanji, Robert Pejchal, Albert Cupo, Umesh Katpally, Rafael S. Depetris, Robyn L. Stanfield, Ryan McBride, Andre J. Marozsan, James C. Paulson, Rogier W. Sanders, John P. Moore, Dennis R. Burton, Pascal Poignard, Andrew B. Ward, Ian A. Wilson

[29]. A) Top and side views of the BG505 SOSIP.664:PGT122 Fab reconstruction at 15 Å resolution with the fitted crystal structures of PGT122 Fab and gp120 core (PDB ID 3DDN [35]) rendered as secondary structure cartoons. The PGT122 Fab is shown in blue (heavy chain) and white (light chain), and the gp120 core is shown in red. B) Top and side views of the BG505 SOSIP.664:PGT122 Fab reconstruction at 15 Å resolution (cyan) and the KNH1144 SOSIP.664:PGT128 Fab reconstruction at 14 Å resolution [29] (light green), with the fitted crystal structure of PGT128:eODmV3 (PDB ID 3TYG [29]) shown as a secondary structure cartoon. The PGT128 Fab is shown in blue (heavy chain) and white (light chain), and the engineered gp120 outer domain (eODmV3) is shown in red. The proposed locations of V1/V2 loops and the V3 loop in the Env trimer, as well as the location of the CD4 binding site, have been labeled. The figure was generated using UCSF Chimera [70].