NMR spectroscopy of ¹⁵N-IgFLNa∆A20–21.

A T₁/T₂ for free (open circles) and migfilin-bound (closed circles) ¹⁵N-IgFLNa∆A20–21. For each domain the average value is shown with a horizontal line. Domain linker region is indicated with a red bar. B Chemical shift perturbations in ¹⁵N-IgFLNa∆A20–21 upon titration with migfilin peptide. Residues corresponding to the β strands are shown with the red bars. Shift changes of ∆δ > 0.1 ppm are shown in the structure of IgFLNa21 in red. Residues with no data are assigned an arbitrary value -0.02 ppm.