Figure_5.tif (877.49 kB)
NLRC4, but not NLRP3 is required for early activation of caspase-1 in macrophages in response to Burkholderia.
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posted on 2014-03-13, 08:22 authored by Antje Bast, Kathrin Krause, Imke H. E. Schmidt, Matsayapan Pudla, Stefanie Brakopp, Verena Hopf, Katrin Breitbach, Ivo SteinmetzProcessing of caspases-1, -9, -7 and PARP was detected by immunoblot in cell lysates of (A) NLRC4-deficient, (B) NLRP3-deficient and respective wild-type BMM infected with B. pseudomallei E8 and B. thailandensis E264 at MOI of 50∶1 at 1.5 hours post infection. One experiment of two performed is shown. non-infected (n.i.).
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immunologymicrobiologyBacterial pathogensGram negativeimmunityImmune defenseinflammationInnate immunityHost-pathogen interactionMedical microbiologyMicrobial pathogenspathogenesisModel organismsAnimal modelsmouseMolecular cell biologycytometryflow cytometrySignal transductionSignaling cascadesApoptotic signaling cascadeMAPK signaling cascadesSignaling in cellular processesApoptotic signalingCell deathInfectious diseasesBacterial diseasesBurkholderia infectionnlrp3activationcaspase-1macrophages
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