NAC attenuates H₂O₂-induced disruption of actin cytoskeleton and cell detachment, but shows limited effect on doxorubicin-induced actin cytoskeleton alteration and cell detachment.

(A) Representative images of rhodamine-phalloidin staining for F-actin (red) and DAPI for nucleus (blue) of WT MEFs treated with 200 μM of H₂O₂ and/or 2 mM NAC for 4 h or with 3 μM of doxorubicin and/or 2 mM NAC for 8 h. Cells showing enriched cortical actin cytoskeleton are indicated with white arrows. Bar, 25 μm. (B) Representative images of bright field photography of WT MEFs treated with 200 μM of H₂O₂ and/or 2 mM NAC for 16 h showing H₂O₂-induced cell detachment. Bar, 400 μm. (C) Both floating and attached cells were collected after 16 h treatment with 200 μM of H₂O₂ and/or 2 mM NAC. Both floating and attached cells were also collected after 16 h treatment with 3 μM doxorubicin and/or increasing concentration of NAC as indicated. Floating cell ratio was expressed as percentage of total cells (floating plus attached cells) under each treatment condition. *P < 0.05 vs. control. ^#P < 0.05 vs. H₂O₂ or doxorubicin only condition.