Figure_4.tif (1.05 MB)
Msp22 shows heme-dependent peroxidase activity.
figure
posted on 2013-05-09, 00:19 authored by Margarita Smidt, Patrick Bättig, Suzanne J. C. Verhaegh, Axel Niebisch, Markus Hanner, Sanja Selak, Wolfgang Schüler, Eva Morfeldt, Christel Hellberg, Eszter Nagy, Urban Lundberg, John P. Hays, Andreas Meinke, Birgitta Henriques-NormarkThe specificity of the heme stain for Msp22 is demonstrated by staining of lysates from the wild type, and gene deletion mutant strains as well as the BBH18 strain transformed with pEMCJH04-KAN-Msp22. Hemoglobin (positive control), BSA (negative control). wt, wild type M. catarrhalis BBH18; wt c*, wild type M. catarrhalis BBH18 transformed with pEMCJH04-KAN-Msp22; msp22Δ, msp22 gene deletion mutant; msp22Δ c* msp22 gene deletion mutant transformed with pEMCJH04-KAN-Msp22. The position of Msp22 is marked with an arrow.
