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Metformin improved insulin mRNA levels, intracellular triacylglycerol (TAG) content, and DNA methylation of Ins1 promoter.
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posted on 2015-02-06, 03:56 authored by Kota Ishikawa, Shin Tsunekawa, Makoto Ikeniwa, Takako Izumoto, Atsushi Iida, Hidetada Ogata, Eita Uenishi, Yusuke Seino, Nobuaki Ozaki, Yoshihisa Sugimura, Yoji Hamada, Akio Kuroda, Keiko Shinjo, Yutaka Kondo, Yutaka Oiso(A) INS-1 cells were cultured in glucose and palmitate for 14 days. (B-D) INS-1 cells were cultured with metformin for 14 days. Intracellular TAG levels (A and D) were examined by ELISA, insulin mRNA levels (B) were examined by real-time PCR, and DNA methylation of the Ins1 promoter (C) was examined by pyrosequencing. All results are means ± SEM (n ≥ 4). Asterisks indicate statistically significant differences (*p < 0.05, **p < 0.01).
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DNA methylation inhibitorDNA methyltransferase activityIns 1 promoterdna methylationinsulin mRNA suppressionrat insulin 1 geneinsEndoplasmic Reticulum StressPalmitate Induces DNA MethylationcAMP response elementrat pancreatic beta cell linepancreatic beta cellsRepresses Transcriptional ActivityMetformin upregulated insulin gene expressioninsulin promoter DNA methylationInsulin Gene Promoterectopic triacylglycerol accumulation
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