Figure_3.tif (5.47 MB)
Mapping of the peptide aptamer binding sites on RasGAP SH3 by AptaPrint.
figure
posted on 2013-02-21, 09:50 authored by Perayot Pamonsinlapatham, Réda Hadj-Slimane, Françoise Raynaud, Marc Bickle, Claudine Corneloup, Audrey Barthelaix, Yves Lepelletier, Perrine Mercier, Matthieu Schapira, Jérôme Samson, Anne-Laure Mathieu, Nicolas Hugo, Olivier Moncorgé, Ivan Mikaelian, Sylvie Dufour, Christiane Garbay, Pierre Colas(A) RasGAP SH3 aminoacid substitutions. (B) AptaPrint assay. 11 LexA fusions with RasGAP SH3 mutants were expressed in EGY48α yeast containing a 8 lexAop-lacZ reporter gene. Peptide aptamer-B112 fusions were expressed in EGY42a yeast. A yeast two-hybrid mating assay was performed. (C) NMR structure of the RasGAP SH3 domain. Residues W317, L313 and D295/7 are located.
