MAb 297-D4 and MAb 144-A8 recognize BAP31 on the cell surface of hESCs.

(A) Immunoprecipitation of biotinylated hESCs with α-BAP31, 297-D4, and 144-A8 antibodies. Immunoprecipitates were detected by western blots with the indicated antibodies or SA-HRP. (B) Flow cytometric analysis of hESCs with anti-TRA-1-60, α-BAP31, 297-D4, or 144-A8 antibodies.