Lipolytic activity and gene annotation of the Pl17.E10 clone.

(A) Clones obtained from the metagenomic approach were cultivated in a Petri dish containing LB media supplemented with 1% (v/v) tributyrin, 1% (w/v) gumarabic, 0.00125% (v/v) chloramphenicol and 0.001% (v/v) arabinose. The plate was maintained at 37°C for three days and for four more days at 4°C. A clear halo was observed around the P117.E10 fosmid clone, evidencing its ability to degrade tributyrin. A detailed physical map of Pl17.E10 is shown, which consists of 25 identified ORFs depicted as arrows according to their location and direction in the fosmid vector. The black arrow indicates ORF16, which encodes the Est16 protein. (B) The characteristics of each ORF were obtained after using ORF Finder and the BLASTP search. The putative function of each ORF and their GenBank accession numbers are also shown.