Leflunomide inhibited the DHODH expression in neuroblastoma.

A. DHODH was commonly expressed in neuroblastomal cell lines.The neuroblastoma cell lines BE(2)-C, SK-N-DZ, and SK-N-F1 were harvested and performed Western blotting assay. α-tubulin was used as a loading control. B.The DHODH Relative mRNA levels were determined by RT-PCR in three neuroblastoma cell lines treated with either DMSO or 100 µM leflunomide for 72 hours. Data represent the average ± SD of at least three independent experiments. Statistical analysis was performed using the 2-tailed.Student t-test. *, p<0.05. C.The DHODH protein expression levels were detected by western blotting in three neuroblastoma cell lines treated with either DMSO or 100 µM leflunomide for 72 hours. α-tubulin as the control.