posted on 2013-02-20, 02:30authored byEdmond F. O’Donnell, Prasad Rao Kopparapu, Daniel C. Koch, Hyo Sang Jang, Jessica Lynne Phillips, Robert L. Tanguay, Nancy I. Kerkvliet, Siva Kumar Kolluri
(A) Protein lysates from A375 cells treated with Vehicle, TCDD, or leflunomide at 100 and 50 µM for 48 and 72 hours were analyzed by Western blot for a panel of cell-cycle regulatory proteins comprising p21, p27, CDK4, CDK6, Cyclin D1, and Cyclin D3. GAPDH was used as a loading control. (B) Expression of p21 mRNA in A375 cells treated with vehicle or leflunomide at 100 and 50 µM was analyzed by qPCR. Data are the mean ± SD, n = 3, # P<0.05, *** P<0.0001.