Kinetics analyses of Est16.

(A) Substrate specificity against p-NP esters of various lengths (C₄ to C₁₆); (B) The effect of pH on Est16 activity against p-NP butyrate; (C) The effect of temperature on Est16 activity against p-NP valerate (■) and octanoate (●); (D) Est16 activity against p-NP butyrate was evaluated in the presence of Ca²⁺, Co²⁺, K⁺, Mg²⁺, Mn²⁺, Ni²⁺, Zn²⁺ and EDTA; (E) The influence of detergents and (F) organic solvents on the enzyme catalytic activity torward p-NP butyrate. In E, the striped bar is triton X-100 and the white bar is tween 20. In F, the black bar is the control, the white bar is DMF and the striped bar is DMSO. In the graphics (A-F), the small letters on the top indicates the significant difference between each condition performed in the experiment, according to ANOVA and Tukey’s test at 5% probablility.