Isolation of human B-cell subsets from bone marrow and tonsils.

A) Overlay of flow cytometry and array data on surface markers used for sorting of the bone marrow B-cell subsets. The contour diagrams show summary of events from the collected B-cell subsets in all samples and dots depict gene expression values (log₂ intensities) in the individual cell samples. B) As in (A), but for tonsillar B-cell subsets and sorting markers. C) Expression profiles of selected genes, dots correspond to group means.