Interaction of E2 with gold standards by HT-GPCA.

(A) Heat maps representing the interactions between the 12 E2 proteins (by columns) and the gold standards (rows). The colour represents Normalized Luminescence Ratio (NLR) obtained by HT-GPCA, from no interaction (black) to strong interactions (light blue). The red rectangles indicate interactions identified in the literature (LCE2-PPI) (B) Heat maps representing the interactions between the 12 E2 proteins (by columns) and the negative random set (rows). (C) Interaction between BRD4 CTD and mutated E2 proteins (16E2I73A and 18E2I77A) tested by HT-GPCA. The results are displayed relative to BRD4 CTD interaction with the wild-type E2 proteins.