Insertion mutation or deletion of the clg1⁺ ORF extends S. pombe CLS.

(A) In the mutant carrying bar codes 4031 and 4033 (Table 1), tandem integration of two vectors (black triangle) was identified in the clg1⁺ ORF and accompanied by a 4-bp deletion (Table 2). (B, C) The CLS of the re-generated mutant bearing the same clg1^- insertion mutation in an isogenic wild type strain (KRP42, B), and a strain bearing a deletion of the clg1⁺ ORF (KRP138, C) were determined in SD + 3% glucose medium (Materials and Methods). Both mutant strains exhibited a longer CLS than the wild type strain (p = 0.0001 for KRP42; p = 0.003 for KRP138). Error bars show the range of the duplicate cultures of each strain.