Increased neutrophil accumulation in adipose tissue of beta2-integrin knock-in mice, but normal macrophage infiltration and differentiation.

(A-B) At the end of the 20-week HF-feeding study, WAT (A) and liver (B) from WT and KI mice were analyzed by flow cytometry to quantify numbers of leukocytes, shown as percentage of live cells (left) and number of cells per g tissue (right). (C) Blood samples were analysed by flow cytometry for leukocyte numbers, shown per 100μl blood. (D-E) F4/80+ macrophages in the adipose tissue (D) and liver (E) were analysed for expression of the M1/M2 macrophage markers, CD86, CD206 and CD11c. n = 10–11. (F) Elastase activity of isolated neutrophils from WT and KI mice, stimulated with LPS or PMA. n = 3. The results are means ± S.E.M. ** P < 0.01; *** P < 0.001.