posted on 2014-03-27, 04:25authored bySharmila Nair, Kristin Michaelsen-Preusse, Katja Finsterbusch, Sabine Stegemann-Koniszewski, Dunja Bruder, Martina Grashoff, Martin Korte, Mario Köster, Ulrich Kalinke, Hansjörg Hauser, Andrea Kröger
Mice were infected intranasally with 5×106 pfu of VSV. A, IRF-1+/−Mx2Luc and IRF-1−/−Mx2Luc transgenic mice were subjected to whole body imaging. Mice were imaged before treatment (0 h) and over time as indicated. Images from a representative mouse are shown. The rainbow scale indicates the number of photons measured per second per cm2 per steradian (sr). B, Serum was collected from WT and IRF-1−/− mice at different time points post infection. IFN-α protein level was determined by ELISA. Data represents mean with SEM of 5–6 mice in each group per time point. Asterisks indicate statistical significance calculated by Mann-Whitney test, ** p<0.005