Figure_3.tif (1.85 MB)
IGF1R expression rescue from miR-223 suppression.
figure
posted on 2013-12-04, 04:20 authored by Li Hui Wu, Qian Qian Cai, Yi Wei Dong, Rong Wang, Bao Mei He, Bing Qi, Chang Jun Xu, Xing Zhong WuA&B. The mRNA expression of IGF1R (A) and Sp1 (B) was monitored by qRT-PCR examination in HeLa or HEK-293T cells transfected with pLL3.7-miR-223, psiCHEK-2-IGF1R3’UTR and decoy nucleotides. The expression levels were normalized to GAPDH which served as the loading control.
C&D. The mRNA expression of IGF1R (C) and Sp1 (D) was measured in SMMC-7721 cells transfected with pLL3.7-miR-223, psiCHEK-2-IGF1R3’UTR and decoy nucleotides.
E&F. The protein levels of IGF1R (C) and Sp1 (D) in SMMC-7721 cells co-transfected with miR-223 and decoy nucleotides (upper), and quantified by densitometry (lower). GAPDH served as an interior loading control. The data are representative from 3 independent experiments. **, P<0.01 .
