posted on 2013-02-22, 08:43authored byXiao-Bing Zhang, Brian C Beard, Katherine Beebe, Barry Storer, R. Keith Humphries, Hans-Peter Kiem
(A) HOXB4-transduced CD34+ cells (lane 1) or FACS-sorted HOXB4GFP+ bone marrow cells from M01035 at 4 mo (lane 2) or 10 mo post-transplantation (lane 3) were subjected to Western blot for detection of HOXB4 expression levels.
(B) HOXB4GFP+ or YFP+ cells from bone marrow or peripheral blood in M01035 were isolated and subjected to Western blot analysis. Protein lysate of peripheral blood cells from a normal control animal was used as a negative control.