Gene expression of glycolytic transcription factors.

Yeast cells were cultured in the absence or presence of 30 µg/ml neomycin for 16 h and harvested. Then RNA was extracted for quantitative RT-PCR analyses. The relative gene expression of RAP1 (A), GCR1 (B) and GCR2 (C) was normalized to the average content per cell of ACT1. Values are expressed as percentages of the average values for the wild-type strain MSS1(P^S). Samples from at least three independent cultures of RNA content and ACT1 for each strain were used in the calculations. *P<0.05, **P<0.01.