Gene expression changes in infected animals treated with Tetracycline.

(A–B) Transcript levels of 6 selected down-regulated genes (A) and 10 selected up-regulated genes (B) from L1 animals grown on S. enterica for 72 hours and then treated with Tetracycline for 24 hours relative to L1 animals grown on S. enterica for 96 hours. Out of the 17 studied genes, only the 16 validated genes are shown. Gray bars represent fold change as determined using qRT-PCR. White bars represent fold change as determined using microarrays. (C–D) Transcript levels of 6 selected down-regulated genes (C) and 10 selected up-regulated genes (D) as determined using qRT-PCR. Black bars represent gene expression changes in L1 animals grown on E. coli for 72 hours and then treated with Tetracycline for 24 hours relative to L1 animals grown on E. coli for 96 hours. Gray bars represent gene expression changes in L1 animals grown on S. enterica for 72 hours and then treated with Tetracycline for 24 hours relative to animals grown on S. enterica for 96 hours. qRT-PCR studies were performed in triplicate. SEM is shown. Statistical significance is indicated (p<0.05: *). (E–G) Transcript levels of 3 selected down-regulated genes (E), 3 selected up-regulated genes (F), and 3 selected housekeeping genes (F) over the infection time course. The expression values of animals grown on S. enterica for 72 hours and then treated with Tetracycline for 24 hours are denoted with open circles. The expression values of animals grown on S. enterica for 96 hours are denoted with an X.