Gelatinase/Collagenase activity of Placental Extract.
(A) Gelatin Zymography - Lane 1, MMP 2; lanes 2, 4 and 6, MMP 2 activation profile in presence of APMA for 0, 1 and 2 hrs; lanes 3, 5 and7, MMP 2 incubated with peptide fraction at 37°C for same duration. (B) Gelatin Zymography - Lane 1 and 2, 0.1 ng collagenase incubated at pH 7.5 for 0 and 20 h at 37°C; lanes 3 and 4, 20 µg peptide fraction with 0.1 ng bacterial collagenase incubated for 0 and 20 h. (C) Collagen zymography. Lanes 1 and 2, 1.0 and 0.1 ng of collagenase; Lane 3, 20 µg of placental peptide fraction. (D) SE-HPLC analysis of collagenase activity of placental extract. a: collagen (Rt = 9.66±0.05 min, n = 5), b: placental extract (15.46±0.08 min), c : collagen treated with the extract at 0 h (major components at 7.3±0.03 and 16.1±0.08 min) and d : 168 h (major components at 7.56±0.01, 8.20±0.03, 8.83±0.001 and 9.60±0.04 min) respectively (n = 5).
