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Epidemiological typing of clinical and environmental P. aeruginosa isolates.

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posted on 2013-02-20, 20:35 authored by Simone Lanini, Silvia D'Arezzo, Vincenzo Puro, Lorena Martini, Francesco Imperi, Pierluca Piselli, Marco Montanaro, Simonetta Paoletti, Paolo Visca, Giuseppe Ippolito

(A) RAPD analysis with primers 208, 228, 241, 272, 275, 277, 287 and ERIC 1, as indicated on top of electropherograms. (B) PFGE analysis of P. aeruginosa isolates. The dendrogram was generated with BioNumerics (Applied Maths) using the unweighted pair-group method with arithmetic averages (UPGMA) and the Dice coefficient. The similarity (%) between isolates is shown at each node of the dendrogram.M is the molecular weight marker (bp); 10 P. aeruginosa from sputum (patient 10); 11 P. aeruginosa from blood culture (patient 11); 13 P. aeruginosa from central venous catheter swab (patient 13); L2 P. aeruginosa from soap; L3 and L4 P. aeruginosa from water.

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