Enhanced proliferation by knockdown of S100b or Sox10 in C3H10T1/2 cells.

(A) mRNA levels of S100b determined by real-time RT-PCR in stable lines of mouse mesenchymal C3H10T1/2 cells retrovirally transfected with S100b, shRNA for S100b, or control GFP. (B) mRNA levels of Sox10 determined by real-time RT-PCR in stable lines of mouse mesenchymal C3H10T1/2 cells retrovirally transfected with Sox10, shRNA for Sox10, or control GFP. (C and D) Growth curves using the CCK-8 assay of stable lines of C3H10T1/2 cells as mentioned above. All experiments were repeated independently three times with data shown as the mean ± SEM. *P<0.05 versus control.