Effects of pegylated hyaluronidase (pegHYAL) and spiperone treatment on HSCs and hematopoietic progenitor cells.

The content of HSCs and hematopoietic progenitor cells in the lung of C57BL/6 mice on the 7th day of the experiment after single dose intratracheal BLM instillation. Membrane receptor's expression of murine HSCs derived from lung were assayed according to the protocol for BD Mouse Hematopoietic Stem and Progenitor Cell Isolation Kit (BD Biosciences, USA). The HSC population taken through a Lin^- selection, is shown gated Sca1⁺ and c-kit⁺, then displayed for CD34^- and CD34⁺. The Lin^- Sca1⁺c-kit⁺CD34^- -cells are LT-HSCs and the Lin^-Sca1⁺c-kit⁺CD34⁺-cells are considered ST-HSCs. Thus, all two of these populations can be readily sorted from one sample. Dot plots are representative figures of three independent experiments with the mean from three independent experiments.

Results are presented as mean and SEM.

*—significance of the difference with the mice, that received intratracheal 0.9% NaCl (P <0.05),

&—significance of the difference with the mice, that received intratracheal BLM and treated with 0.9% NaCl (P<0.05), t test was used.

Effects of pegylated hyaluronidase (pegHYAL) and spiperone treatment on HSCs and hematopoietic progenitor cells.