Effects of Gln on mitochondrial membrane potential stability and ATP level.

H9C2 cells were treated as described in Fig 3. (A). Representative images of JC-1 confocal images of each group; the red image detected in the left panel represents JC-1 aggregates, and the green image represents JC-1 monomer; the blue image represents DAPI, right panel shows the merged image. (B) Mean ± S.D. of the changes in radiometric JC-1 fluorescence for each group as indicated. (C) Cellular ATP levels of each group were detected. * P < 0.05 compare with the control group; # P < 0.05 compare with the 1 mM Gln and 4 mM Gln groups. Data are expressed as the mean ±S.D. of 3 independent experiments. a.u. = arbitrary units.