Effect of testosterone or testosterone in combination with β-estradiol on 17βHSD7 gene expression in HepG2 cell line and pig primary hepatocytes.

The data were normalized with HPRT housekeeping gene. Treatments: 1T = 1 nM testosterone, 1T +10E2 = 1 nM testosterone +10 nM β-estradiol, 10T = 10 nM testosterone, 10T +10E2 = 10 nM testosterone +10 nM β-estradiol, 100T = 100 nM testosterone, 500A = 500 nM testosterone. Each bar represents an average value for four independent experiments. Each measurement was done in triplicate. Error bars represent standard errors for means. The effect of treatments to 17βHSD7 gene expression were compared with control, and bars with superscript differ significantly (P<0.05). “Control” cells were kept under same conditions as the experimental cell lines but without added steroids. Incubation time was 24 hr. * indicates p<0.05.