Effect of Gln on cytosolic GSH, GSSG and intracellular ROS.

H9C2 cells were exposed to HG+H/R with different concentrations of Gln (1, 4, or 16 mM). (A) Cytosolic GSH, (B) GSSG, and (C) the GSH/GSSG ratio in each group were detected. (D) Representative images of DCFH flow cytometry of each group. (E) Mean DCFH fluorescence intensities are presented as percentage relative to the control value. (F) Representative images of DHE flow cytometry of each group. (G) Mean DHE fluorescence intensities are presented as percentage relative to the control value. These data are expressed as the mean ± S.D. of 3 independent experiments. * P < 0.05 compare with the control group, # P < 0.05 compare with the 1 mM Gln and 4 mM Gln groups, a.u. = arbitrary units.