posted on 2014-01-31, 04:27authored byJie Li, Stephen C. Pak, Linda P. O’Reilly, Joshua A. Benson, Yan Wang, Tunda Hidvegi, Pamela Hale, Christine Dippold, Michael Ewing, Gary A. Silverman, David H. Perlmutter
Separate monolayers were incubated for 48 hours in the absence or presence of Flu or CBZ. Drug was added to the medium daily. Cells were then harvested, homogenized and the cell homogenates separated into soluble and insoluble fractions. The fractions were analyzed by immunoblot for AT (top) as well as loading controls, GelCode Blue (middle) and GAPDH (bottom). A, HTO/Z cell line expressing mutant ATZ; B, Densitometric analysis of 4 separate experiments in HTO/Z cell line. Mean +/− standard error is shown with error bars. Asterisks denote a statistically significant difference (p = 0.0029 for insoluble ATZ; p = 0.0292 for soluble ATZ).