Effect of Flu on steady state levels of ATZ in genetically engineered HeLa HTO/Z cell line.

Separate monolayers were incubated for 48 hours in the absence or presence of Flu or CBZ. Drug was added to the medium daily. Cells were then harvested, homogenized and the cell homogenates separated into soluble and insoluble fractions. The fractions were analyzed by immunoblot for AT (top) as well as loading controls, GelCode Blue (middle) and GAPDH (bottom). A, HTO/Z cell line expressing mutant ATZ; B, Densitometric analysis of 4 separate experiments in HTO/Z cell line. Mean +/− standard error is shown with error bars. Asterisks denote a statistically significant difference (p = 0.0029 for insoluble ATZ; p = 0.0292 for soluble ATZ).