Table_2.xls (5.5 kB)

Early embryonic development of parthenogenetically activated oocytes aged under the effect of the H₂S donor.

dataset

posted on 2015-01-23, 02:42 authored by Tereza Krejcova, Miroslava Smelcova, Jaroslav Petr, Jean-Francois Bodart, Marketa Sedmikova, Jan Nevoral, Marketa Dvorakova, Alena Vyskocilova, Ivona Weingartova, Veronika Kucerova-Chrpova, Eva Chmelikova, Lenka Tumova, Frantisek Jilek

Oocytes were cultivated 48 hours to the metaphase II and then divided into 3 groups (see table). Control group (MII) was parthenogenetically activated immediately (without any exposure to prolonged cultivation). Other groups were exposed to prolonged cultivation (aging) for 24 hours in modified M199 medium supplemented with a H2S donor (Na₂S.9H₂O; 0μM, 150μM, and 300μM) and then parthenogenetically activated with calcium ionophore (25 μM, 5 min) combined with 6-dimethyl aminopurine (2 mM, 2 h). Subsequently, oocytes were cultured in NCSU 23 medium for 168 hours (7 days). The ratio of cleaved embryos was evaluated after the first 48 hours of culture.

^a,b,c,d Statistically significant differences in type of embryo stage between individual treatments (in columns) are indicated with different superscripts (P<0.05).