ELT-2 regulates the expression of specific genes during recovery.

(A) Representation factors of previously studied data sets that overlap with genes altered during recovery at the 96-hour time point. (B) Percentage of down- and up-regulated recovery genes with at least one TGATAA site in the 1.5 kb sequence upstream of the transcriptional start site. Randomly selected gene sets are included as controls. (C–D) Transcript levels of 3 selected down-regulated genes (C) and 5 selected up-regulated genes (D) as determined using qRT-PCR. Gray bars represent gene expression changes in control fer-1(b232ts) young adult animals exposed to S. enterica—GFP for 36 hours and then treated with Tetracycline for 24 hours relative to control fer-1(b232ts) young adult animals grown on S. enterica—GFP for 60 hours. Checkered gray bars represent gene expression changes in fer-1(b232ts) elt-2(RNAi) young adult animals exposed to S. enterica for 36 hours and then treated with Tetracycline for 24 hours relative to control fer-1(b232ts) young adult animals exposed to S. enterica—GFP for 36 hours and then treated with Tetracycline for 24 hours. qRT-PCR studies were performed in duplicate. SEM is shown.