Downregulation of ICln by siRNA and 4.1R membrane localisation.

(A) Exemplificative images of HEK cells co-transfected with the ptdTOMATO-N1vector and scrambled (ctrl) or ICln siRNA (ICln). The samples were immunolabelled with an anti-4.1R antibody (4.1R) or anti-ICln antibody (ICln). Scale bar 20 µm. (B) Western blot of total protein extracts from HEK cells co-transfected with ICln siRNA (ICln) or scrambled siRNA (ctrl) and the fluorescent tdTomato protein. The histograms show the mean OD value of the ICln signal normalised for the corresponding GAPDH signal (n = 4). The values are percentages of the control. **p<0.01.