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Double immunofluorescence staining for VEGF-C with α-SMA (myofibroblast), ED1 (macrophages), and with podoplanin (lymph vessels).

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posted on 2013-02-19, 19:48 authored by Saleh Yazdani, Fariba Poosti, Andrea B. Kramer, Katarina Mirković, Arjan J. Kwakernaak, Menno Hovingh, Maartje C. J. Slagman, Klaas A. Sjollema, Martin H. de Borst, Gerjan Navis, Harry van Goor, Jacob van den Born

Most podoplanin positive lymph vessels were observed close to tubules (mostly proximal) which showed VEGF-C expression at 12 weeks (arrows). After double staining for macrophages and myofibroblast, most of these interstitial cells were negative for VEGF-C. (Bar = 100 µm).

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