Fig_7.tif (1.02 MB)

Distribution of bucl genes among Burkholderia spp. select agents by PCR.

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posted on 2015-09-10, 03:45 authored by Beth A. Bachert, Soo J. Choi, Anna K. Snyder, Rita V. M. Rio, Brandon C. Durney, Lisa A. Holland, Kei Amemiya, Susan L. Welkos, Joel A. Bozue, Christopher K. Cote, Rita Berisio, Slawomir Lukomski

Presence of bucl genes was assessed by PCR on (A) a collection of genomic DNA from 25 B. pseudomallei and 16 B. mallei strains, as well as (B) in control strains of B. thailandensis, B. cepacia, B. cenocepacia, and B. multivorans; selected bucl genes 5, 13, 14, and 16 are shown. (C) Detection and separation of selected bucl amplicons generated from the B. pseudomallei reference strain K96243 by traditional 2% agarose gel electrophoresis (left) or by capillary gel electrophoresis (right). Electropherogram generated by capillary gel electrophoresis with phospholipid nanogel matrix shows separation of amplicons over time. Amplicon sizes: bucl5, 216 bp; bucl13, 214 bp; bucl14, 178 bp; and bucl16, 123 bp. M, 50-bp DNA ladder. PCR data shown in Panel A for 25 Bp strains come from two merged gel images.