Figure_5.tif (1.57 MB)
Derivation of peripheral neural cells, glia, and melanocytes from hNCCs.
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posted on 2014-12-02, 03:41 authored by Makoto Fukuta, Yoshinori Nakai, Kosuke Kirino, Masato Nakagawa, Kazuya Sekiguchi, Sanae Nagata, Yoshihisa Matsumoto, Takuya Yamamoto, Katsutsugu Umeda, Toshio Heike, Naoki Okumura, Noriko Koizumi, Takahiko Sato, Tatsutoshi Nakahata, Megumu Saito, Takanobu Otsuka, Shigeru Kinoshita, Morio Ueno, Makoto Ikeya, Junya ToguchidaA) Neuronal differentiation of 201B7-derived hNCCs. Cells were stained with an antibody against peripherin (red) and Tuj-1 (green). B) The glial differentiation of 201B7-derived hNCCs. Cells were stained with an antibody against GFAP. Scale bar, 50 µm. C) Melanocyte differentiation of 201B7-derived hNCCs. The mRNA expression levels of MITF and c-KIT genes were shown as a relative value using the value in 201B7-derived hNCCs and 201B7 as 1.0, respectively. Average ± SD. N = 3, biological triplicates.
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Defined Media Neural crest cellsgskegfcell populationtgfinduction protocolmolecule compoundscraniofacial skeletonFGF 2Mesenchymal Stromal CellsDifferentiation propertiesPluripotent Stem Cellsmultipotent mesenchymal stromal cellsbasal mediumgrowth factormaintenance stepshNCCcdmSmall Molecule Compoundsskin pigmentationmsccell typesNeural Crest Lineage10 passagesgene expression profilesescdifferentiation characteristicsculture conditionsncc
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