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Depletion of SIRT3 impairs Myogenin, MyoD and SIRT1 expression.
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posted on 2014-12-09, 02:44 authored by Waed Abdel Khalek, Fabienne Cortade, Vincent Ollendorff, Laure Lapasset, Lionel Tintignac, Béatrice Chabi, Chantal Wrutniak-CabelloWestern-blot analyses of Myogenin, MyoD, Sirt1 and Tubulin in C2C12-LucshRNA (shCTL) and C2C12-SIRT3shRNA (shSIRT3) cells at the indicated states (Proliferation, P; Cell Confluence, C and 3 days after the induction of differentiation D3). Results are normalized relatively to Tubulin protein levels and expressed as the mean ± SD of three separate experiments. ANOVA main effect: ## P<0.01, ### P<0.001 vs. shCTL cells. Post-hoc significance: *P<0.05, **P<0.01 and ***P<0.001 vs. proliferating myoblasts for each cell type. §P<0.05, §§P<0.01 and §§§ P<0.001 vs. shCTL cells at the same stage.
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nadmyoblast fusion indexSIRT 3 protein expressionSirt 3C 2C cellsMyoblast Differentiation Sirtuin 3C 2C myoblast differentiationC 2C myoblastsrosrnaReactive oxygen speciesSIRT 3 deacetylasesatpSIRT 3 expressionmanganese superoxide dismutaseMitochondrial Activitycitrate synthase protein expressionpgcSIRT 3 modulates mitochondrial activitymyoblast differentiationTroponin T protein expression
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