Fig_4.tif (756.78 kB)
DSF analysis of avian H5N1 rNA.
figure
posted on 2015-08-17, 02:58 authored by Marua Prevato, Ilaria Ferlenghi, Alessandra Bonci, Yasushi Uematsu, Giulia Anselmi, Fabiola Giusti, Sylvie Bertholet, Francois Legay, John Laird Telford, Ethan C. Settembre, Domenico Maione, Roberta CozziThe thermostabilizing effect of Ca2+ ions binding to rNA was detected by DSF in the presence of Sypro orange. The graph shows fluorescence intensity vs temperature for increasing amount of Ca2+, 0.079–20 mM, in 25 mM Tris pH 8, 150 mM NaCl buffer.
