Fig_5.tif (4.29 MB)
Competition assay between VHHs.
figure
posted on 2015-08-12, 03:39 authored by Lorena Garaicoechea, Andrea Aguilar, Gabriel I. Parra, Marina Bok, Stanislav V. Sosnovtsev, Gabriela Canziani, Kim Y. Green, Karin Bok, Viviana ParreñoThe images show immunofluorescence staining of Vero cells expressing NoV VP1 from Norwalk strain (a) or MD2004 strain (b). Ten μg of an unlabeled VHH together with 1 μl of Alexa Fluor 568 labeled VHH were incubated on the fixed cells. A decrease in the fluorescent signal indicates a competitive binding with the labeled VHH.
