Comparison of interacting residues with Me-Man in binding pocket of Artocarpin, Banlec, Jacalin and IPO.

(A) The interacting residues of Artocarpin (PDB: 1J4U) with Me-Man are Gly15, Thr91, Gly137, Asp138, Leu139, and Asp141. Sticks of Artocarpin are purple. (B) The interacting residues of Banlec (PDB: 1X1V) with Me-Man are Gly15, Val86, Gly129, Asp130, Phe131, and Asp133. Sticks of Banlec are yellow. (C) The interacting residues of Jacalin (PDB: 1WS5 chain A) with Me-Man are Gly1, Tyr78, Gly121, Tyr122, Trp123, Asp125. Sticks of Jacalin are orange. (D) Stereo view of the binding pocket of Artocarpin, Banlec, Jacalin, and IPO with Me-Man. The structures of Artocarpin (in purple), Banlec (in yellow), Jacalin (in orange) and IPO (in blue) were superimposed. The binding pocket of IPO is labeled with the interacting residues Gly21, Tyr97, Gly141, Trp142, Tyr143 and Asp145, with Me-Man in blue. The binding mode of Me-Man in IPO is compared with the other 3 lectins and are shifted outside by hydrogen binding with Gly21. The moving distance is about 1.5 Å (indicated by an arrow) from Gly15 of Artocarpin and Banlec to Gly21 of IPO.