Comparison of bacterial community structure as recovered in T-RFLP fingerprinting and pyrotag libraries.

Results are shown for selected aquifer sediment DNA extracts (replicate b from each year). For pyrotag ‘fingerprints’, the abundance of dominating assembled contigs is illustrated via T-RFs predicted in silico along with the total number of reads contributing to that contig. (*) illustrates the 282 bp pseudo T-RF of specific Spirochaete populations detected as in vivo T-RF only.