Characterization of exosomes released from differentiated subtypes of macrophages.

Exosomes were isolated from conditional media of differentiated macrophages and examined for the presence of specific markers by RP-PCR (A) and western blot (B). Expression of Arg1 and CD206 mRNA and protein (markers for M2 subtype) was detected in exosomes originated from M2 macrophages, but not in those secreted from M1 macrophages. In contrast, expression of iNOS mRNA and protein was detected in exosomes released from M1 macrophages, but not in those secreted by M2 macrophages. TSG101 was used as house-keeping protein for exosomes. Values are means ±SEM (N = 4), and p<0.05 compared with the expression levels in Mo macrophages.