Characterization of ρ0 cells.
A Multiplex PCR analysis of mitochondrial DNA. The nuclear and mitochondrial genes (18S rDNA: 229 bp and D-Loop: 436 bp, respectively) were coamplified and visualized by gel electrophoresis. Agarose gel (1.5%), lane 1 and 6: GeneRuler™ 100 bp plus DNA Ladder, lane 2: PC-3 wild type, lane 3: PC-3 ρ0 EtBr, lane 4: PC-3 ρ0 9B4, lane 5: no template. B PCR analysis of EcoRI gene sequence in PC-3 ρ0 9B4 cells. Different EcoRI gene sequences were amplified by PCR from PC-3 ρ0 9B4 genomic DNA utilizing the primer pairs listed in Table 2. Agarose gel (1.5%), lane 1 and 17: GeneRuler™ 100 bp Plus DNA Ladder, lane 2–4: PC-3 ρ0 9B4, vector DNA (encoding mitochondrial targeted restriction endonuclease, 500 pg) and no template, primer pair A, lane 5–7: primer pair B, lane 8–10: primer pair C, lane 11–13: primer pair D, lane 14–16: primer pair E. C Cell growth analysis of PC-3 cells. Calculation of doubling time of PC-3 cells in media with uridine (dark grey bars) and without uridine (light grey bars). Total cell number was measured every 24 h over a period of six days and cell doubling was estimated using exponential regression. The presented data are means ± SD from four independent experiments. *P<0.05, **P<0.01, ***P<0.001.
