posted on 2013-10-01, 02:37authored byCyrille Mionnet, Isabelle Mondor, Audrey Jorquera, Marie Loosveld, Julien Maurizio, Marie-Laure Arcangeli, Nancy H. Ruddle, Jonathan Nowak, Michel Aurrand-Lions, Hervé Luche, Marc Bajénoff
CD21cre-RFP chimeras were untreated (left panel) or injected (right panel) with an emulsion of CFA/PBS in the ears. Three weeks later, recipients were injected with a cohort of CMFDA-labeled Wt-deficient B cells (green) and Celltrace violet–labeled CXCR5-deficient B cells (violet). One day later, ear draining LNs were sectioned, stained for B220 (blue) expression, and imaged by confocal microscopy. RFP+ cells appear in red. Data are representative of two different experiments (three mice per experiment).