CSC induced the expression of Wnt5a and increased colony forming capacity in non-CSC exposed 1799 cells.

(A) 1799 cells treated with 10% CSC for 8 days and further cultured in normal growth medium. CSC-transformed clones of 1799 cells were isolated and used to profile the expression of Wnt5a using quantitative RT-PCR. (B) Selected R10 and R11 clones were examined for the colony-forming efficiency with or without a PKC inhibitor (Gö: 2.5 µM Gö6o76). Colonies were fixed in methanol and stained with 0.1% crystal violet. The data are representative of three experiments. Quantification of data means the average number of colonies (>20 pixel²) per dish from the triplicate. Bars denote standard error. Significant differences (*: p<0.05, **: p<0.005) from control as determined by a Student's t test.