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CRASP-3 and CRASP-5 bind diverse complement proteins.
figure
posted on 2013-02-20, 22:50 authored by Corinna Siegel, Teresia Hallström, Christine Skerka, Hannes Eberhardt, Barbara Uzonyi, Tobias Beckhaus, Michael Karas, Reinhard Wallich, Brian Stevenson, Peter F. Zipfel, Peter KraiczyBinding of equimolar amounts of CFH, CFHR1, CFHR2, CFHR5 (33 µM) to immobilized CRASP-3, CRASP-5, ErpX, and ErpQ (5 µg/ml) was analyzed by ELISA. Bound CFH or CFHR proteins were detected with either goat CFH polyclonal antiserum or mouse CFHR1 monoclonal antiserum (JHD 7.10), which reacts with all the three CFHRs. Data represent the means and standard errors from three separate experiments.
